Enzymatic Route for the Production of Biofuels

Enzymatic Route for the Production of Biofuels CHAPTER 1 : INTRODUCTION 1.1 Background of Study Many compounds are currently produced through reactions that use chemical catalysts or expensive experimental conditions. However, this practice is aggressive to the environment, generating effluents with high cost of treatment, and consuming too energy. Enzymatic catalysis appears thus to reduce the energy demand contributing to a reduction in by-product formation. However, the acquisition cost of enzymes is still high, making difficult the access of biocatalyst to industries. An alternative to this financial barrier is immobilize the enzyme (ZHENG et al., 2012). Constant efforts are being made to improve the enzyme’s activity, efficiency, reproducibility and stability during industrial processes (Wang et al. , 2010). Enzyme immobilisation is confinement of enzyme to a phase (matrix/support) different from the one for substrates and products. Inert polymers and inorganic materials are usually used as carrier matrices. Apart from being affordable, an ideal matrix must encompass characteritcs like inertness, physical strength, stability, regenerability, ability to increase enzyme specificity/ activity and reduce product inhibition, nonspecific adsorption and microbial contamination (Singh, 2009). Immobilization generates continous economic operations, automation, high investment/capacity ratio and recovery of product with greater purity (D’Souza, 1998). There are several factors affecting the immobilization processes such as adsorption, covalence bound, entrapment and cross- linking. During the initial years of the development in the field of immobilized enzymology, researchers used to find only the advantage of the immobilized enzymes in comparison to their soluble / free counterparts. Advantages of immobilized versus soluble enzymes included comparative studies in pH profile, various denaturing agents organic solvents, and temperature. Now recently during the last couple of decades, immobilized enzyme technology has advanced into and ever- expanding and multidisciplinary fields to analyze clinical, industrial and environmental samples. Examples of the recent developments and used of immobilized enzymes in different fields such as in medicine, antibiotic production,drug metabolism,food industry, biodiesel production and bioremediation. 1.2 Problem Statement The use of enzymatic route for production of biofuels is growing up due the mild reaction conditions. In fact of that, we must find a way to reduce the cost to ensure the experimental that are highly cost can be carried out and despite of that, immobilize enzyme is the best way that can be used. In addition, it also can be use multiple or repetitive in a single batch of enzyme. It become more economics as it also has the ability to stop the reaction rapidly by removing the enzyme from the reaction solution. By finding this solution, so that there are no problem arise to continuously used it as a biocatalyst. 1.3 Objectives To provide an alternative way to reduce the experimental cost by using immobilized enzyme To study the differences on the enzyme activity when using pH and mass media To develop the easiest method that can be use by the others as it does not need a professional qualitification. CHAPTER 2 : LITERATURE REVIEW 2.1 Enzyme First and foremost, enzymes are the active components in the cells, where they induce chemical transformations. Besides, enzymes are large, complex macromolecules, consisting largely of protein and usually contain a prosthetic group (one or more metal atoms). It also acts as biocatalyst that catalyzes all chemical conversions needed for the system’s survival and reproduction (Buchholz et al., 2005; Idris et al., 2008). Besides, enzyme also act as catalyst to boost up the rate of chemical reaction by factor up to more than and result to the reduction of activation energy compared to uncatalyzed reaction (Buchholz et al., 2005) as shown in figure 3.1. On top of that, enzymes have unique characteristics for instance high activity, selectivity, and specificity which permit their performance in their complex process. Figure 2.1: Free energy diagram for an uncatalyzed and enzyme-catalyzed reaction (Buchholz et al. , 2005) Enzymes are specific to the types of reactions that they catalyze. In addition, they are more stable and easier to handle than the original microorganisms from which they were isolated. On top of that, enzymes play an important role in biochemical analysis and it is widely used in many industrial processes especially in food production and many more. On the other hand, properties of most enzymes are dependent on pH of their environment. Dependence of catalytic activity on the concentration of hydrogen ions in solution is given by protonable groups. These groups are the parts of active site of enzymes and also are present in substrate molecules. Thus, the reaction between the enzyme and the substrate is dependent on the degree of protonation. That is the reason why majority of enzymes are catalytically active in specific interval of pH values. If the pH value is higher or lower than pH optimum of certain enzyme, catalytic activity of enzyme decreases. pH optimum is value of pH at which the activity of an enzyme reaches the maximum. Changing of pH values of reaction medium is one way how to regulate the activity of enzyme (Vodrazka et al. , 2001). Even though enzymes give benefits in many application, there are some restrictions should be stated. Enzymes are excellent reactions catalysts but without any improvement thus in pure state, they are not very suitable for use in reactors in industrial scale. On the other hand, enzymes may be instable due to spontaneous oxidation, self digestion, or denaturation and they work properly only on natural substrates and under physiological conditions. In addition, they are soluble in water and also in organic solvents and can be strongly inhibited by excess amount of substrates, product formed during enzyme-catalyzed reaction and however by certain by-products of the reaction. 2.2 Immobilized Enzyme Enzyme immobilization can be described as the attachment of free or soluble enzymes to different types of support. This process turns the soluble enzyme into insoluble form by interaction with specific type of support. As a result, the mobility of the enzyme is reduced or lost (Khan et al . , 2010). Immobilized means enzyme has been confined or localized so that it can be reused continuously (Ramachandra et al . , 2002). Immobilized enzyme are currently the subject of considerable interest because of their advantages over soluble enzymes or alternative technologies, and the steadily increasing number of application for immobilized enzymes (Tisher et al. , 1999). Numerous methods for achieving the immobilization of lipases are available, each involves a different degree of complexity and efficiency. There are various methods used to date are adsorption, ionic bonding, covalent bonding, cross linking, entrapment, and encapsulation (Ramachandran et al. , 2002). 2.3 Support Chen et al (2011) studied the effect of hydrophobicity of membranes used as support for the immobilization of lipase by covalent bond in the activity and stability of the enzyme, and obtained better results when used more hydrophobic membrane. Studies claim that the region surrounding the active site of lipases is hydrophobic, and because of that, they recognize hydrophobic surfaces as similar to their natural substrate and undergo interfacial activation (FERNANDEZ- LAFUENTE et al. , 1998). Zhou (2012) showed that, in general, substrates with high specificity had greater surface area for adsorption capacity, while the more hydrophobic again, the best results are attributed to the improved interfacial activity of the lipase. It is possible to predict, taking into account the objectives in view, the selected substrate should have high surface area, be thermally stable, chemically durable, resistant to contamination and reasonable cost (KANDASAMY et al. , 2010). Because of all these cha racteristics was chosen support material, the MCM41, whose family is characterized by having a hexagonal arrangement of uniform pores and well- defined size, with linear channels constructed with a silica matrix (KRESGE et al. , 1992). 2.4 Methods of Immobilization Many review and books on the immobilization of enzyme have been published during the last two decades (Bahulekar et al. , 1993 Kennedy Cabral, 1983). In the course of the last decades, numerous methods of immobilization a variety of different materials have been developed. However, different types of immobilization may have different effects on the enzyme activity or stability, is not always predictable at forehand (Arroyo et al. , 1999; Cao, 2011). Therefore, advantages and disadvantage have been described in Table 3.1 below. On the other hand, it is known that any type of immobilization method has the potential to stabilize the enzyme relative to their native form (Cao, 2011).Basically, there are four ways to immobilize enzyme onto surfaces as explain below : Adsorption This method immobilization is the connection between enzyme and support without any chemical modification. Besides, this is the most used method for immobilization due to some advantages such as low cost, no chemical additives required and high activity (Fukuda et al. , 2011). Covalence This method of immobilization is based on covalent bound between enzyme and carrier. During the catalyst action, the interaction between enzyme and support is very strong which makes the enzymes very stable. Instead having an advantage, covalent binding also has some disadvantage as well like the reaction conditions are complicated, there is a high risk of loose of enzyme activity during the process, and some coupling reagents are toxic (Fukuda et al. , 2011). Entrapment This method of immobilization is process of capture of the enzyme into the inner hollows of some specific matrix or into microencapsules of polymer. Thus, an enzyme inside of the matrix is not attached to the polymer and its free diffusion is only restrained (Fukuda et al. , 2011). Cross Linking This method of immobilization is the interaction between enzyme, coupling reagent, and carrier will form the three dimensional network structures. This method lead to strong interaction between the protein and the carrier, but activity of the immobilized enzyme is low. That is the reason why cross linking is often combined with adsorption to achieve higher immobilization efficiency (Fukuda et al. , 2011). Table 2.1 :The advantages and disadvantages of immobilization method 2.5 Advantages of Immobilized Lipase Although lipases presently account for no more than 3% of all enzymes produced worldwide, the use of immobilized lipases for the modification of melted fats and oils is currently a subject of expanding interest (Ramachandran et al. , 2002). This interest is due in part to the fact that the use of lipases has the potential to be more cost effective when enzyme are employed in immobilized rather than in free form (Ramachandran et al. , 2002). Besides, there are several reasons to use immobilized enzymes such as easy separation of enzyme from the product and reuse of the enzyme. Easy separation of the enzyme from the product simplifies enzyme applications and permits reliable and efficient reaction technology. Enzyme reuse provides a number of cost advantages (Tisher Kasche, 1999). Furthermore, the use of immobilized lipases leads to a decrease in potential for contamination of the product via residual lipases, thus avoiding the need for downstream thermal treatment (Ramachandra et al. , 2002). The immobilization of enzyme is a useful tool to meet cost targets and has a number of technological advantages (Tisher Kasche, 1999). Immobilization also permits multiple uses of the lipases and often enhances its thermal and chemical stability, thus leading to predictable decay rates. It also enhance oppurtunities for better control of both the process and product quality. In fact the increment costs of using an immobilized biocatalyst in a continuos process are more than 20 times lower than with a traditional one (Ramachandra et al. , 2002). Owing to the world wide variety of properties of individual enzyme species and the varying requirements of reaction technology for the target compounds, it is necessary to exploit the wealth of methods and techniques of immobilization (Tisher Kasche, 1999). The main disadvantage of immobilization can be loss of activity due to immobilization, limitation in substrate’s diffusion, possible leakage of the biocatalyst from the support (Ramachandra et al . , 2002). 2.6 Classification of Enzyme Enzyme can be classified into six categories according to the reaction catalyzed. Every enzyme is defined by a unique set of 4 numbers. The first number denotes the reaction they catalyze, the second number indicates chemical structure that are changed in the process, the third shows the properties of the enzyme involved in the catalytic reaction and the forth implies the running number (Buchholz et al. , 2005). Table 3.2 shows the six group of enzyme and the type of reaction catalyzed (Knez et al. , 2001). Table 2.2: Classification of enzyme and the reaction catalyzed (Knez et al. , 2001) 2.7 Lipase from Candida Rugosa Lipase from Candida rugosa sp. is one of the most attractive commercially available lipases for complete hydrolysis of triacylglycerols because it has ability to liberate all types of acyl chains, despite of their positions in the triacylglycerols (Virto et al. , 1994). Candida rugosa sp. is one of the most extensively studied microorganisms by biotechnologists due to its powerful lipase ( E.C. 3.1.1.3) production capacity. In addition, Candida rugosa sp. lipase has extensive substrate specificity which provides successfully used in a variety of hydrolysis and esterification reactions. Furthermore, it is possible used in synthesis of several pharmaceuticals (Benjamin Pandey, 1998) due to its high stereoselectivity and regioselectivity. The yeast of Candida rugosa sp. secreted several extracellular lipases and they differ in terms of molecular weight, carbohydrate content, isolectric point specificity (Pernas et al. , 2000). Besides, it is commonly used for several streoselective esterification reactions in organic medium under mild reaction conditions (Abdul Rahman et al. , 2005). CHAPTER 3: MATERIALS AND METHODS Materials and Equiment Table 3.1: List of chemicals and equipment Experimental design 3.2.1 Preparation of Buffer solution In conducting this experiment, at pH 7.0 and 8.0, three buffer solutions of 50 mM of Potassium phosphate at pH 6.0 are made. Preparation to remove organic part on support 1 g of MCM 41 support are mixed with 10 mL of 10% HNO3 v/v and are strirring for 30 minutes. The solution are filtrate and the successive washes of water and buffer solution are removed. 3.2.3 Dilution for the p-NP (p- nitrophenol) 0.0014g of the reagent are weighed and are dissolve in a buffer to complete 100mL in volumetric flask. Dilutions of p-NP are made in the same buffer and are analyze on a spectrophotometer with absorbance 410nm using blank reaction buffer solution. Determination of enzymatic activity The substrate are using lipase and p-NFL (p-nitrophenillaurate). 0.018 g of the substrate are dissolve in 1 mL of DMSO and buffer are add until 100 mL flask are complete. For preparing the lipase solution, 0.1 g of the enzyme solution are dissolve in a 100mL of the buffer solution and subsequently performing various dilutions. Lipase solution with substrate are place in 2mL of cuvette at 40s for contact time. The pH variation is due to buffer at pH 6, 7 and 8. Immobilization process A beaker containing 20mL of p-NPL is place on magnetic stirrer is use for immobilization. To know the influence of the mass of support for immobilization, different mass of MCM 41 are add. The samples is collect and is being analyze by spectrophotometer at interval 10 minutes. CHAPTER 4 : EXPECTED RESULTS The expected results for this study are: The increasing of the enzyme activity as the concentration of p- NF concentration is add and at what concentration it direct to to the enzymatic activity. By using the enzyme concentration ( lipase enzyme), the curve of enzymatic activity is expect to fall. The time for adsorption between the enzyme and support at pH 7 is just short if smaller mass support is use. The pH 7 is most convenient than pH 8 for the adsorption of the mass support of MCM 41. CHAPTER 5 : CONCLUSION The use of enzymatic route for the production of biofuels can be overcome by using the immobilization of enzyme. The influence of pH and mass media used will effect enzyme activity. The experiments that carry out at pH 6 will show a fall absorbance with the increasing of the enzyme concentration. This experiments also show that the lipase is best at pH 7 and pH 8 where the results are better at pH 7.0. As the optimal immobilization at a very short time where it shows high affinity of the enzyme for support.The mass support also shows best in a very small amount of adsorbed enzyme. This also shows that the cost benefits with the use of support, which will be smaller.

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Genocides of the World: Armenia â€Å"Armed roundups began on the evening of April 24, 1915, as 300 Armenian political leaders, educators, writers, clergy and dignitaries in Constantinople (present day Istanbul) were taken from their homes, briefly jailed and tortured, then hanged or shot (United Human Rights Council).† That was the ending of some of the thousands upon thousands of lives lost during the Armenian genocide. Some say this genocide never was an actual genocide, but when that many people die I don’t know how it can’t be considered one. Some of the deaths where slow and dragged on until the victim’s body could no longer take it, while others where quick and easy. This is the Armenian Genocide. We decide our future, and we must not let anger get the best of us. The Ottoman Empire was angered by the Armenians, and now is responsible for the horrible attacks and murders towards the Armenian people. Now, all they can do is pretend it didn’t happen. At its peak, The Ottoman Empire consisted of most of Southeast Europe, North Africa, and almost all of the Middle East (United Human Ri...

Relative Frequency And Effects Of Disasters Environmental Sciences Essay

IntroductionHarmonizing to India catastrophe study 2011, 14 States and one Union Territory of India had experienced at least one catastrophe during the twelvemonth 2011. The harm occurred in footings of figure of human lives lost was 1432 and figure of cowss perished was 6266. In entire 6,84,901 houses were damaged and 16.28 lakh hectares of cropped country were affected. 1 Rank Catastrophe Year of happening Entire figure of people affected 1 Drought 1987 300,000,000 2 Drought 2002 300,000,000 3 Drought 1972 200,000,000 4 Flood 1993 128,000,000 5 Drought 1965 100,000,000 6 Drought 1982 100,000,000 7 Drought 2000 50,000,000 8 Flood 2002 42,000,000 9 Flood 1975 34,000,000 10 Flood 1982 33,500,000 Source- EM-DAT: The OFDA/CRED International Disaster Database www.em-dat.net – Universite Catholique de Louvain – Brussels – Belgique Table1.2 Top 10 Natural Disasters in India during 1900-2012 in footings of economic harm Rank Catastrophe Year of happening Damage ( 000 US $ ) 1 Flood 1993 7,000,000 2 Flood 2006 3,390,000 3 Flood 2005 3,330,000 4 Earthquake 2001 2,623,000 5 Storm 1999 2,500,000 6 Flood 2004 2,500,000 7 Flood 2005 2,300,000 8 Storm 1990 2,200,000 9 Flood 2009 2,150,000 10 Flood 2010 1,680,000 Source- EM-DAT: The OFDA/CRED International Disaster Database www.em-dat.net – Universite Catholique de Louvain – Brussels – Belgique Droughts and inundations were the catastrophes which affected maximal figure of people in India. Table1.1 shows the top 10 natural catastrophes in India in footings of entire figure of people affected during 1900-2012. In footings of economic harm it was once more the inundations which had caused immense economic losingss to India. Table1.2 shows the top 10 natural catastrophes in India in footings of economic harm during 1900-2012.1.2 CatastropheSo what is a catastrophe? Let ‘s discuss about it. Disaster is defined as happening of calamity in any country which may be due to natural or manmade causes. It can happen as an accident or due to negligence and may ensue in loss of human life and/or cattle life and/or harm to belongings. It may besides take to debasement of environment. Community of the affected country normally do n't hold the capacity to get by with the state of affairs without external support. 2 Catastrophes can be classified into two wide types: natural catastrophes and manmade catastrophes. The High Power Committee on catastrophe direction in India, constituted in 1999, has identified 31 different catastrophes which can be categorized into five major groups. 3 I. Water and clime related catastrophes two. Geological catastrophes three. Chemical, industrial and atomic catastrophes four. Accident related catastrophes v. Biological catastrophes1.2.1 Disaster directionDisaster direction is necessary non merely for prompt response during catastrophe but for bar of it and for decrease of hazard and badness of any catastrophe. It is a uninterrupted and incorporate procedure of planning, organizing, organizing and implementing different activities and steps. It includes readiness, appraisal of magnitude of effects, prompt response such as emptying, deliverance and alleviation. It besides includes rehabilitation and reconstruction.2 A typical catastrophe direction rhythm has six elements ; the pre-disaster stage comprises of bar, extenuation and readiness while the post-disaster stage comprises of response, rehabilitation, Reconstruction and recovery. 4 Traditionally, direction of any catastrophe worldwide, focussed on immediate deliverance and alleviation operation, so as in India. After the Great Famine of 1876-1878 in India, the Famine Commission was constituted in 1880. The Famine Relief Code was besides adopted. 5 Relief sections were set up under British regulation for exigencies during catastrophes. After independency, catastrophe direction in each province was looked after by Relief Commissioners, under Cardinal Relief Commissioner. Their function were limited to distribution of alleviation stuff in the affected countries. 3 Consequent upon announcement of the decennary 1990-2000 as International Decade for Natural Disaster Reduction ( IDNDR ) by the general assembly of the United Nations 6 and the universe conference on natural catastrophes decrease at Yokohama, Japan in 1994, Government of India constituted a High Powered Committee in August, 1999. 7 The High Power Committee gave its recommendations in October 2001 including a bill of exchange of the catastrophe direction measure and suggested for the constitution of National Disaster Management Authority. 3, 5 After Odisha Super Cyclone ( 1999 ) and Gujarat Earthquake ( 2001 ) , India changed its catastrophe direction attack from reactive to proactive. 8 After the super cyclone, the Government of Odisha established the Odisha State Disaster Management Authority ( OSDMA ) . 51.2.2 Relative frequence and effects of catastrophesWorld meteoric organisation statistics shows that harm caused by natural catastrophes during 1963-2002 was worst for inundations ( Flood-32 % , Tropical cyclone-30 % , Droughts-22 % , Earthquakes-10 % and other disasters-6 % ) . 3 78.4 % of all catastrophe events worldwide occur due to hydro-meteorological causes and 47.94 % of all catastrophe deceases worldwide are due to hydro-meteorological catastrophes from 1900 to 2009. 3 Children and adult females are the most vulnerable group as 85 % of the deceases during catastrophes are of adult females and kids. 31.3 FloodFlood is defined as ‘the status that occurs when H2O overflows the natural or unreal confines of a watercourse, river, or other organic structure of H2O, or accumulates by drainage over low-lying countries ‘ . 9 Flood can happen in a little localised country due to heavy rainfall over a sustained period of clip and the attendant drainage job. Flash implosion therapy occurs when it rains excessively rapidly, by and large for less than six hours. But river inundations are normally of longer continuance as it may last a hebdomad or more and in some instances for months together doing more harm to human lives and farm animal. Coastal inundations are caused by tsunami, heavy costal rainfall and tidal action.10 River inundations are expected in some geographical countries. Peoples by and large welcome inundations as they provide rich dirt for cultivation and H2O for assorted intents. But flood at an unexpected graduated table and with inordinate frequence causes harm to lives, farm animal and the environment. Matter of concern is that, there is addition in the frequence and strength of inundations in many parts of the universe including India due to current clime change.111.3.1 Flood in IndiaThe Indian sub-continent is extremely vulnerable to assorted types of natural catastrophes like drouths, inundations, cyclones, temblors, and landslides etc. India is one of the 10 worst catastrophe prone states of the universe. 3 Among all these natural catastrophes that occur in the state, river inundations are the most frequent and frequently the most annihilating. In India, 40 million hectares of land which is about one-eighth of the entire country of the state is prone to inundations. Flood occurs in 23 out of entire 35 provinces and brotherhood districts in the state. 3 To command inundation in the state, The National Flood Control Program was launched in India in 1954. 31.3.2 Flood in OdishaOdisha province which is situated in the east seashore of India is one of the most vulnerable Indian provinces to climate change.12 It is located between 170 48 ‘ N and 220 35 ‘ N latitudes, and 810 47 ‘ E and 870 32 ‘ E longitudes. 1 The chief rivers of Odisha are Mahanadi, Brahmani, Baitarani, Budhabalanga, Subarnarekha, Rushikulya etc. These rivers are perennial with sulky flow throughout the dry season. With the oncoming of monsoon they swell threateningly and deluge big countries. 1 Odisha experiences terrible inundations in about every two old ages. 131.3.3 Flood in Kendrapara territory of OdishaKendrapara territory is one of the coastal territories of Odisha. After separation from Cuttack territory as a separate territory in 1993, Kendrapara has faced terri ble inundations in 1994, 1995, 1997, 1999, 2001, 2003, 2006, 2007, 2008, 2009 13and late in 2011.14 Harmonizing to India catastrophe study 2011, Kendrapara territory was severely hit by inundation in 2011. All the nine development blocks in the territory were affected. 473 small towns of 116 Gram Panchayats and two Urban Local Bodies ( ULBs ) were affected. Entire figure of population affected were 507145 ( Third highest after 625897 in Puri territory and 526923 in Cuttack territory ) with 13 human casualties ( Highest in the province ) . 27000 houses were damaged ( Second highest after Puri territory ) including kuchha and pucca houses. 11.3.4 Health effects of inundationFloods are frequently considered the most frequent and dearly-won of all natural catastrophes in footings of human agony and economic loss.15 Health impacts of inundation vary between populations and these depend on the physical exposure of population, single every bit good as community readiness and the type and continuance of flood event. The immediate wellness effects of inundation can be submerging, hurt, acute asthma, tegument roseolas, stomach flu, and respiratory infections. The mid-term effects of inundation are infected lesions, toxic condition, catching diseases, and famishment. The long-run wellness effects of inundation can be disablement, hapless mental wellness and malnutrition. 16 Approximately two-thirds of the flood deceases can be attributed to submerging. This implies that other one-third human deaths are due to causes other than submerging, such as physical injury, bosom onslaught, fire, C monoxide toxic condition and electrocution.17 In inundation state of affairss, there are ever increased opportunity of transmittal of diseases like cholera, cryptosporidiosis, infantile paralysis, rotavirus, enteric fever and paratyphoid, particularly in countries if the community does non hold entree to safe imbibing H2O and sanitation.18 Common mental upsets like anxiousness, depression and posttraumatic emphasis upset are common after a nerve-racking event of an exceptionally baleful nature like terrible inundation. 18 These may attest with symptoms like upseting memories, turning away of fortunes associated with the stressor, sleep perturbations, crossness and deficiency of concentration.18 Loss of cherished ownerships in inundation can hold much more impact than fiscal losingss. 19 One survey found a four times increase in unwellnesss among people whose places were flooded compared with those whose places were non flooded.20 Referrals to infirmaries were more than double from the flooded families than not flooded families for the old ages following the inundations in Europe. 21 One community degree survey in rural Odisha, India shows that exposure to inundations is associated with long-run malnutrition. Children who are exposed to inundations during their first twelvemonth of life show higher degrees of chronic malnutrition compared to the kids who are non exposed to deluge during their infancy.221.4 Public wellness system and inundationPublic wellness system should stay prepared to supply exigency services to the community as the demand may increase all of a sudden when inundation strikes the community. 23 but public wellness systems face tonss of jobs in pull offing the inundation state of affairs.1.4.1 ProblemsFlood can hold impact on the public wellness attention systems in two ways. The direct impact is due to structural harm to wellness installations and the secondary impact is through other direction jobs. 24 Scarcity of resources reduces the ability of the wellness system to react efficaciously. 25 Public wellness systems besides face jobs in presenting services due to population supplanting and power failure. Population supplanting leads to herding and sanitation job around the impermanent colonies. This may take to outbreak of diseases. 26 Water intervention workss may halt working due to power failure job during inundations. This increases the hazard for waterborne diseases. Power failure job besides affects proper operation of wellness installations such as care of cold concatenation. 26 Disease surveillance in affected countries is of import to understand the impact of inundation on diseases. But acquiring accurate and timely information during inundation is often disputing. 26 Healthcare instantly after inundation is frequently delivered by many bureaus and organisations. Coordination among them at that clip becomes a challenge. 261.4.2 ReadinessIn general inundation readiness steps include building of dikes, land usage planning, watershed direction, inundation prediction and warning system, inundation eventuality planning and readiness of community for self-defense activity and capacity edifice programmes. 11 Public and the public governments ever focus on structural intercessions that modify and control the velocity and the force of flood.10 Primary wellness Centres under public wellness system are the chief wellness attention establishments in rural countries to cover with wellness impacts of catastrophes. 27, 28 Poor people are more likely to be affected than the wealthier, when public wellness establishments are affected. 29 To cut down the wellness impacts on a population significantly, wellness system should hold information on impending inundation. 30 Flood readiness planning for public wellness system should be an on-going procedure. It should embrace all stakeholders so that the corporate wisdom can be utilised to cut down the impact of inundation, to take necessary action during the inundation event and to take up proper rehabilitation and recovery activities. 31 but it is a common fact that public wellness planning processes in most of the states including India are based on premises and guesss instead than grounds. 271.4.3 CommunityNational Disaster Management Authority ( NDMA ) has advised the Ministry of Panchayati Raj and Rural Development to turn to the concerns of catastrophe direction in the preparation of representatives of the Panchayati Raj Institutions and local organic structures. Women Self Help Groups ( SHGs ) , Anganwadi workers and adult females voluntaries are playing a lead funct ion in catastrophe direction readiness. Accredited Social Health Activist ( ASHA ) workers are besides easing first assistance and hunt and deliverance preparation for big figure of people. 8 So community should be cognizant about their exposure and strength. They are themselves the first respondents to any type of catastrophes. Education and preparation of the general community in first assistance and resuscitation decidedly helps in salvaging many lives before formal medical assistance reaches the catastrophe site. Evaluations of developing plans on first assistance and resuscitation have shown good consequences. Contingency plans and extenuation programs for inundation become successful merely when the planning procedure involves all the community members and it take into history the bing societal constructions and dynamics.32 It has been estimated that, 80-90 per centum of wellness attention demands in the first 24 hours after inundation strikes the community can be managed by trained voluntaries from the community itself. This besides reduces the work load on the public wellness system. 331.4.4 Public wellness workersEnrolling and retaining human resources in public wellness system is an all clip challenge. There is acute deficit of human resources in rural countries and it is felt clearly during catastrophes like inundation. 34 But Training of available human resource in flood direction can cut down the service spread during inundation well. 35 Training and capacity edifice of wellness forces is one of the most of import elements of catastrophe readiness program of public wellness system. Trained work force shows assurance in managing exigency state of affairss. Evaluation of preparation and capacity edifice activities are normally done by comparing pre-tests and post-tests36 but the existent rating of competence and effectivity occurs as the jeopardy strikes the community. Training besides helps to detect inadequacies in accomplishments, determination pickings pattern and information systems. 37 Training should hold theory Sessionss based on success narratives elsewhere in the universe and mock Sessionss supplying accomplishments to cover with the practical job. The most of import thing is that there should be refresher preparations on a regular basis with regular updating of cognition. 38 There should be more capacity edifice activities for community degree wellness workers as they are the first contact points for the community in exigency state of affairss like inundation. These activities should be based on recognized scientific attacks but adapted to the local culture.391.5 Public wellness system in OdishaCommissioner-cum-secretary of section of wellness is the administrative caput of the section and studies to the wellness curate. Nine managers and the drug accountant of Odisha study straight to the commissioner-cum-secretary. These managers are Mission Director, National Rural Health Mission ( NRHM ) ; Project Director, Odisha State AIDS Control Society ( OSACS ) ; Dir ector, Medical Education and Training ; Director, Acharya Harihar Regional Cancer Centre ( AHRCC ) ; Director, Family Welfare ; Director, Health Services ; Director, Public Health ; Director, State Institute of Health and Family Welfare ( SIHFW ) ; Director, Indian System of Medicine ( ISM ) and Homeopathy. Commissioner-cum-secretary is besides helped by secretaries at assorted degrees such as particular secretary, extra secretary, joint secretary, deputy secretary and under secretary. ( Annexure-1 ) District wellness disposal is headed by head territory medical officer. Public wellness system below territory degree has been described in the subdivision 3.1 ( Health system in the survey territory ) of consequence chapter. Table1.3 gives an thought about the figure of public wellness attention establishments in Odisha which provide services to entire population of 41,947,358 ( Rural 34,951,234 and Urban 6,996,124 ) in Odisha.40 Medical College and Hospitals 3 District Hospitals ( 30 territories + Capital Hospital, Bhubaneswar & A ; Rourkela General Hospital ) 32 Sub-Divisional Hospitals 26 Community Health Centres 377 Other Hospitals 79 Primary Health Centres 1228 Sub-Centres 6688 Ayurvedic Hospitals 2 Ayurvedic College & A ; Hospitals 3 Ayurvedic Dispensaries 619 Homoeopathic College & A ; Hospitals 4 Homoeopathic Dispensaries 561 Unani Dispensaries 9 Source- Annual Activity Report 2011-12, Health and Family Welfare Department, Government of Odisha.1.5.1 Public wellness system response to inundation in OdishaDirectorate of Public Health in Odisha has been created by a declaration of Department of Health & A ; Family Welfare in 2009. Disaster direction is one of the chief activities of the board of directors. 14 The State unit of Integrated Disease Surveillance Project ( IDSP ) becomes the province control room during inundation. During 2011 inundation, 135 medical alleviation squads were deployed and 482 Medical Relief Centers were opened in the inundation affected districts.141,73,374 packages of ORS and 52,74,613 Halogen tablets were distributed. 11.6 Rationale for the surveySuccessful direction of wellness impacts of inundation depends on the coordination of assorted sections and bureaus with the wellness section, cooperation from the community and leading of the wellness section. This can merely be achieved by bettering the ap prehension of wellness hazards in local scenes and of the societal and cultural qualifiers of those risks.17 Research on inundation hazards and response has mostly focused on economic sciences, support and agribusiness. Very few researches have given attending to wellness dimension of inundation. 41 A study of primary wellness centres in Jagatsinghpur territory of Odisha, surrounding territory of Kendrapara was done instantly after 2008 inundation. The aims were to measure the readiness and functional capacity of primary wellness centres in the territory for inundation response. Pretested questionnaire was used to interview medical officers merely. Health workers and other back uping staffs were non interviewed. 42 No mentions of survey conducted in Kendrapara territory which tries to understand the wellness hazards of community and wellness staffs, inter and intra wellness section work kineticss, outlook and cooperation of community and community based organisations in flood state of affairss were found. This survey is an effort to understand these local wellness system kineticss which can be utilized to alter the policy for better readiness of wellness system to pull off and extenuate the inauspicious wellness impacts of inundation in Kendrapara territory.1.7 Aims of the surveyaˆ? To analyze the major jobs encountered by public wellness workers in presenting the services after inundation aˆ? To analyze the readiness of public wellness workers for pull offing inundation state of affairs aˆ? To happen out the capacity spreads of public wellness workers in covering with inundation state of affairs

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